Journal: Cells

Article Title: Propiconazole-Induced Testis Damage and MAPK-Mediated Apoptosis and Autophagy in Germ Cells

doi: 10.3390/cells14201624

Figure Lengend Snippet: PRO exposure affects the testicular germ cell. ( A ) Body weight and ( B ) Body weight gain from each experimental group. Data are presented as mean ± SD. ( C ) Testis weight and ( D ) Seminiferous tubular diameter were measured from each group. Data are shown as mean ± SD. ** p < 0.01 ( E ) Gene expression levels of Sall4 , Piwil 1 , Nanos2 , and Dazl from each experiment group. The graph shows mean ± SD with log 2 scale. n = 5, ** p < 0.01. ( F ) Hematoxylin and eosin staining of testis from each group, showing immunostaining of SALL4 in testes. Scale bar = 100 μm. ( G ) The number of DDX4+ and SALL4+ cells from seminiferous tubules. At least 50 tubules were scored for each testis (five to seven biological replicates). The graph is shown as mean ± SD, and significance levels between Ctrl and treated are presented as asterisks *** p < 0.001. ( H ) Protein expression levels of SALL4 in testis from each group. The graph shows relative protein expressions normalized to β Actin ( n = 3). ** p < 0.01.

Article Snippet: SALL4 , Santa Cruz Biotech , SC-101147 , 1:200 (1:1000).

Techniques: Gene Expression, Staining, Immunostaining, Expressing